SangerAlignment Level

SangerContig Level

SangerRead Level

Basic Information:

  • Output Directory : /Users/camilogarcia/Documents/Teaching/BiologyCourses/BI0487/Lectures/biocomp-book/chs-challenges-demos/data/sanger-processed/rpoB/SangerAlignment/321_WT_rpoB/321_WT_rpoB_2_R
  • Input Directory : data/sanger-seqs/rpoB/.
  • Input Filename : 321_WT_rpoB_2_R.ab1
  • Read Feature : Reverse Read

DNA Seqence

Primary Sequence

Secondary Sequence

Phred Quality Scores

Amino Acids Sequence

Amino Acids Sequence frameshit 0

Amino Acids Sequence frameshit 1

Amino Acids Sequence frameshit 2

Quality Trimming

  • Trimming Method: Method 1 ‘Logarithmic Scale Trimming’
  • M1 Trimming Cutoff: 1e-04
  • Raw Sequence Length: 1391
  • Trimmed Sequence Length: 950
  • Trimmed Start Point: 25
  • Trimmed End Point: 975
  • Raw Mean Quality Score: 40.6951833
  • Trimmed Mean Quality Score: 56.3073684
  • Raw Min Quality Score: 2
  • Trimmed Min Quality Score: 17
  • Remaining Read Ratio: 68.3 %

Chromatogram

  • Signal Ratio Cutoff: 0.33
  • Base Number Per Row: 100
  • Height Per Row: 200
  • Show Trimmed Region: TRUE 
## Chromatogram saved to /Users/camilogarcia/Documents/Teaching/BiologyCourses/BI0487/Lectures/biocomp-book/chs-challenges-demos/data/sanger-processed/rpoB/SangerAlignment/321_WT_rpoB/321_WT_rpoB_2_R/Chromatogram.pdf in the current working directory

Click Here to Open Chromatogram in New Page




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